Effect of cycloheximide treatment on mRNA transcript levels.
| Treatment | ΔCt | ΔΔCt | % Normal transcript | P value |
|---|---|---|---|---|
| R171X (CHX −) | 1.225 | 1.435 | 37 | 0.002 |
| R171X (CHX +) | 0.375 | 0.585 | 67 | 0.185 |
| I414G415ATCG→CCA (CHX −) | 18.68 | 18.89 | <0.01 | 0.001 |
| I414G415ATCG→CCA (CHX +) | 0.18 | 0.39 | 76 | 0.603 |
| P291fsinsC (CHX −) | 5.98 | 6.19 | 2 | 0.001 |
| P291fsinsC (CHX +) | −0.38 | −0.17 | 112 | 0.776 |
| R229P (CHX −) | 0.775 | 0.985 | 51 | 0.03 |
| R229P (CHX +) | 1.245 | 1.455 | 36 | 0.003 |
| G207D (CHX −) | 0.02 | 0.23 | 86 | 0.580 |
| G207D (CHX +) | −0.58 | −0.37 | 129 | 0.656 |
ΔCt refers to the difference between the crossing point for the C allele and the T allele. ΔΔCt refers to this figure normalized to the controls. Median ΔCt values and SDs are shown for all mutations with and without cycloheximide treatment. The calculated levels of the mutant transcript are given together with their P values as determined by Mann-Whitney test.