TABLE 2

Sequences of each primer pair and their location in sequences cited in GenBank

	Sense primer	Antisense primer	Amplicon size (bp)	GenBank accession no.
Conventional RT-PCR primers
HK-I	CAACAGTGTGAAGTCGGCCT	CTCTGTCAAACTCGGTTCGG	175	NM_012734
GLUT-3	TGTTCGCTGTTACTGTTGCC	CACACATAACGACCAGAGCG	170	NM_017102
MCT-1	ATGTTGTCCTGTCCTCCTGG	CTCCGCTTTCTGTTCTTTGG	172	D63834
MCT-2	TTCTTGCTGTCTGTGATGGC	AACACGACTATCCCACTGGC	395	X97445
LDH-A	GGCAACCCTTAAGGAAAAGC	CTTCCAAGACATCCACCAGG	314	NM_012595
LDH-B	TAAGGAAGAACAGGTCCCCC	TGAAGATGTTCACGTTTCGC	167	NM_017025
Lepr-b	CCATTCCCAGCTCACTGTCT	GAACAGGATTGAAACTGGGG	137	D84551
GAD	CTGCTTCCAGCTAAGAACGG	GGATCTGCTCCAGAGACTCG	198	NM_017007
GFAP	CCATGGTCCTACCAGACACC	ACACAGCCAGGTTGTTCTCC	443	L27219
β-Actin	CCGTAAAGACCTCTATGCCA	AAGAAAGGGAAAAGCCA	300	V01217
Multiplex RT-PCR primers for first and second amplifications
GK
1^st	CCTGAAGAAGGTGATGAGCC	AGGGAAGGAGAAGGTGAAGC	373	NM_012565
2^nd	GATGCAGAAGGAGATGGACC	GGTTCCTCCCAGGTCTAAGG	139
GKRP
1^st	GTCGTCATAGGCATCTCTGTGG	CTGGTTGGTGAGTTCATCCTTC	624	NM_013120
2^nd	ATCCTGAGGACATTTGAGCG	TGTGGATGCACTCTACTCCG	172
GLUT2
1^st	AAGGATCAAAGCCATGTTGG	GGAGACCTTCTGCTCAGTCG	502	NM_012879
2^nd	TGCTGGAAGAAGCGTATCAG	GGCCAAGTAGGATGTGCCAG	206
GLUT4
1^st	TCTTTGAGATTGGTCCTGGC	TACTGGGTTTCACCTCCTGC	312	NM_012751
2^nd	GGTTTCTCCAACTGGACCTGTAA	ACGGCAAATAGAAGGAAGA	95
SGLT-1
1^st	CGGGCAGCTCTTTGACTACATCCA	AAGGCGGGGTTCAGGCAAAATA	661	D16101
2^nd	CTTCTGGGGACTGATTCTCG	CGCTCTTCTGTGCTGTTACG	255
Kir6.2
1^st	CGTCACAAGCATCCACTCCT	CACCTGCATATGAATGGTGG	328	X97041
2^nd	CCTTTTCTCCATCGAGGTCC	TGAAGATGAGGGTTTCTGCC	188
Kir6.1
1^st	AGCCACTGACCTTGTCAACC	GGAGTCATGAATTGCACCTT	402	D42145
2^nd	CATAGTGATTCTCGAGGGCG	AAGGTTTCTCGTCCAGCTCC	212
SUR-1
1^st	TGGACCCAGAGAAGAAATGC	ACAAAGGAGGCAAAGACGC	506	L40624
2^nd	TGAAGCAACTGCCTCCATC	GAAGCTTTTCCGGCTTGTCC	182
SUR-2
1^st	AGTGGCAACAACCTGAAACC	AATGTGGCTAAACCACTGCC	377	D83598
2^nd	TTCTAACCTATCCATGGGCG	TTCTAACCTATCCATGGGCG	218
INS-R
1^st	CTGAAGGAGCTGGAGGAGTC	GATTTCATGGGTCACAGGGC	426	M29014
2^nd	GCCACTACACCCACACTTCC	CTTAGCTTCAGGCATGGTCC	233

GFAP, glial fibrillary acidic protein.