TABLE 5

Effects of 6 days of glucose, fructose, and galactose feeding on expression of sympathetically related genes in IBAT and WAT of male rats

DietGlucoseFructoseGalactoseP (diet)
n6666
Body wt (g)174.4 ± 3.6a191.5 ± 3.4b184.4 ± 4.5a,b174.7 ± 3.5a0.011
IBAT
 UCP1 (cycles)20.9 ± 0.5a16.6 ± 0.3b16.1 ± 0.2b19.6 ± 0.5a<0.0001
 GLUT4 (cycles)24.9 ± 0.4a21.2 ± 0.3b21.6 ± 0.2b25.5 ± 0.5a<0.0001
 β3-AR (cycles)25.5 ± 0.224.8 ± 0.225.4 ± 0.425.4 ± 0.4NS
 Leptin (cycles)27.3 ± 0.4a27.6 ± 0.2a,b28.0 ± 0.4a,b28.7 ± 0.2b0.023
 AGT (cycles)26.9 ± 0.2a25.8 ± 0.3b25.8 ± 0.2b26.9 ± 0.3a0.003
 RPL19 (cycles)20.8 ± 0.4a19.0 ± 0.2b19.2 ± 0.2b21.2 ± 0.4a<0.0001
Retroperitoneal fat
 GLUT4 (cycles)26.8 ± 1.1a22.9 ± 0.3b23.1 ± 0.6b28.0 ± 1.3a0.001
 β3-AR (cycles)27.9 ± 1.1a,b25.2 ± 0.2a25.0 ± 0.6a28.7 ± 1.2b0.009
 Leptin (cycles)30.5 ± 1.0a24.7 ± 0.3b24.4 ± 0.5b30.4 ± 0.9a<0.0001
 AGT (cycles)29.2 ± 1.1a26.0 ± 0.7a,b25.7 ± 0.8b29.7 ± 0.8a0.005
 RPL19 (cycles)21.2 ± 0.719.7 ± 0.519.5 ± 0.621.1 ± 0.8NS
Epididymal fat
 GLUT4 (cycles)25.0 ± 0.823.4 ± 1.423.1 ± 1.023.1 ± 1.2NS
 β3-AR (cycles)26.5 ± 0.826.5 ± 0.925.6 ± 0.724.9 ± 0.8NS
 Leptin (cycles)27.8 ± 0.4a25.6 ± 0.6a,b25.1 ± 0.6b27.6 ± 0.3a0.004
 AGT (cycles)25.3 ± 0.824.6 ± 0.724.2 ± 0.824.8 ± 0.9NS
 RPL19 (cycles)21.1 ± 1.020.2 ± 0.819.5 ± 0.919.8 ± 0.9NS
  • Data are means ± SE. “Cycles” refers to the number of PCR cycles required to achieve a predetermined level of amplification for the reactions. The number of cycles to the threshold value is inversely related to the relative abundance of a specific message in 0.25 μg of RNA extracted from the tissue. Samples from all 24 animals were run in triplicate in a single assay. Variables with the same superscript are not statistically different; those with dissimilar superscripts differ significantly by post hoc analysis at P < 0.05.