TABLE 6

Effects of 6 days of fructose feeding on NE levels and gene expression in WAT of intact and sympathectomized male rats

Intact
Sympathectomized
P*
DietFructoseDietFructoseRxDiet
n7777
Body wt (g)253.1 ± 1.1248.1 ± 3.3236.7 ± 3.4241.7 ± 3.90.001NS
Retroperitoneal fat
 Tissue wt (mg/pad)197 ± 19297 ± 33205 ± 41400 ± 65NS0.002
 Tissue NE (ng/pad)60.4 ± 5.767.9 ± 3.617.5 ± 1.317.2 ± 0.8<0.0001NS
 UCP1 (cycles)28.2 ± 1.225.2 ± 1.231.2 ± 0.329.2 ± 0.70.0010.012
 GLUT4 (cycles)23.5 ± 0.821.1 ± 0.824.4 ± 0.423.4 ± 0.40.0190.012
 β3-AR (cycles)27.4 ± 0.526.9 ± 0.628.3 ± 0.128.0 ± 0.10.020NS
 Leptin (cycles)26.9 ± 0.524.4 ± 0.628.1 ± 0.525.3 ± 0.30.038<0.0001
 AGT (cycles)25.7 ± 0.424.6 ± 0.727.2 ± 0.226.1 ± 0.20.0020.025
 AGTR1A (cycles)20.6 ± 0.220.8 ± 0.321.1 ± 0.121.2 ± 0.10.022NS
 RPL19 (cycles)19.6 ± 0.419.1 ± 0.720.8 ± 0.320.4 ± 0.10.011NS
Epididymal fat
 Tissue wt (mg/pad)661 ± 38795 ± 29586 ± 39777 ± 61NS0.001
 Tissue NE (ng/pad)54.6 ± 4.247.6 ± 4.77.4 ± 1.38.9 ± 0.6<0.0001NS
 GLUT4 (cycles)22.2 ± 0.321.8 ± 0.722.6 ± 0.622.0 ± 0.5NSNS
 β3-AR (cycles)27.2 ± 0.227.5 ± 0.427.5 ± 0.227.6 ± 0.1NSNS
 Leptin (cycles)23.1 ± 0.322.6 ± 0.424.0 ± 0.322.6 ± 0.5NS0.019
 AGT (cycles)23.0 ± 0.323.5 ± 0.624.6 ± 0.523.7 ± 0.30.066NS
 AGTR1A (cycles)19.7 ± 0.119.8 ± 0.120.0 ± 0.120.0 ± 0.10.048NS
 RPL19 (cycles)18.4 ± 0.318.9 ± 0.419.3 ± 0.219.0 ± 0.2NSNS
  • Data are means ± SE. *The interaction term (Rx × Diet), included in all analyses, was uniformly NS. “Cycles” refers to the number of PCR cycles required to achieve a predetermined level of amplification for the reactions. Samples from all 28 animals were run in triplicate in a single assay. AGTR1A, angiotensin receptor1A.