TABLE 1

Fatty acid composition of total cellular lipids

Fatty acidMIN6 controlMIN6 palmitatePR3 controlPR3 palmitate
16:024.7 ± 3.435.2 ± 3.625.7 ± 2.028.0 ± 2.2
16:1n73.0 ± 1.19.8 ± 1.94.0 ± 1.37.4 ± 1.3
18:022.8 ± 3.319.0 ± 1.419.5 ± 1.916.5 ± 1.4
18:1n921.3 ± 1.414.9 ± 0.622.6 ± 0.819.8 ± 0.4*
18:1n77.4 ± 0.95.1 ± 0.59.5 ± 0.811.1 ± 1.9
20:4n66.4 ± 1.16.3 ± 0.48.6 ± 0.27.7 ± 0.3
16:0/18:1 ratio0.88 ± 0.181.78 ± 0.200.80 ± 0.070.91 ± 0.08
  • Data are percentage of total fatty acids in the total lipid extracts and are means ± SE of three independent experiments done in duplicate. Cells were treated for 48 h in medium supplemented with 2.3% BSA alone (control) or 1 mmol/l palmitate/2.3% BSA (palmitate).

  • *

    * P < 0.01 and

  • P < 0.05 compared with palmitate-treated MIN6 cells.