TABLE 3

Transsulfuration flux in isolated hepatocytes

Transsulfuration flux (nmol · mg dry wt−1 · 30 min)
5 weeks11 weeks
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l-[1-14C] methionine1.00 ± 0.251.08 ± 0.051.18 ± 0.211.08 ± 0.15
l-[1-14C] methionine + betaine0.91 ± 0.320.87 ± 0.12*0.96 ± 0.15*0.86 ± 0.13*
  • Data are means ± SD; n = 3 for 5 weeks and n = 5 for 11 weeks. Hepatocytes were incubated with 0.1 mmol/l l-[1-14C] methionine ± 0.3 mmol/l betaine to determine the transsulfuration flux.

  • * Significant difference from the corresponding incubations without betaine (P < 0.05).