TABLE 1

List of primers

TargetForwardReverseSize
UCP-2AACAGTTCTACACCAAGGGCAGCATGGTAAGGGCACAGTG471
β-ActinGCAATGCCTGGGTACATGGTGGGTCGTACCACAGGCATTGTGATGG492
Acetyl CoA carboxylaseGGGACTTCATGAATTTGCTGATTCTCAGTTGTCATTACCATCTTCATTACCTCAATCTC728
Fatty acid synthaseCACAGATGATGACAGGAGATGGTCGGAGTGAGGCTGGGTTGAT205
PPAR-γ coactivator 1CGCAGCCCTATTCATTGTTCTCATCCCTCTTGAGCCTTTC364
Sterol regulatory element–binding protein 1cATCGGCGCGGAAGCTGTCGGGGTAGACTGTCTTGGTTGTTGATGAGCTGGAGCA116
LeptinTCCAGAAAGTCCAGGATGACACCACATTTTGGGAAGGCAGG212
Carnitine palmitoyl transferase 1ATTCTGTGCGGCCCTTATTGGATTTTGCCTGGGATGCGTGTAGTGT395
Long-chain acyl-CoA dehydrogenaseGCTGCCCTCCTCCCGATGTTATGTTTCTCTGCGATGTTGATG258
UCP-1CACCTTCCCGCTGGACACCCCTAGGACACCTTTATACCTAATG91
Hormone-sensitive lipaseCCTACTGCTGGGCTGTCAACCATCTGGCACCCTCACT142
PPAR-γTTGACAGGAAAGACAACGGAGAGCAGAGTCACTTGGTCATT246
GLUT4TCTCCAACTGGACCTGTAACTCTGTACTGGGTTTCACCTC221
Muscle-type phosphofructokinaseAGATGGGTGCTAAGGCTATGTTTTGAGGATTGGCCTCAGC218
Muscle-type pyruvate kinaseCATTGCCGTGACTCGAAATCCATGGTGTTGGTGAATCCAG225
Hexokinase ICGTGGTGCAAAAGATCCGAGCTGCTCTTAGGCGTTCGTAG243
Hexokinase IITCTCAGATCGAGAGTGACTGCGTCTCATGCATGACTTTGG272
ARCAGCATTATTCCAGTGGATGGGGGCACTTGCACAGAGATG274
UCP-1 promoterTCCATTGGCCTCAAACCCTATGAGAGGCGTGAGTGCAAGAACAAAAGG3,850