TABLE 1

Metabolic and blood pressure data in WT and iNOS knockout mice fed a standard or high-fat diet at 4 and 8 weeks of feeding

4 weeks8 weeks
Body mass (g)
    WT, standard24.1 ± 0.329.1 ± 0.4
    WT, high-fat27.3 ± 0.4*38.4 ± 0.4*
    iNOS KO, standard22.0 ± 0.827.2 ± 1.1
    iNOS KO, high-fat27.9 ± 0.536.6 ± 1.3
Epididymal fat pad (mg)
    WT, standard11.7 ± 1.112.0 ± 0.3
    WT, high-fat24.4 ± 1.5*33.6 ± 0.9*
    iNOS KO, standard11.3 ± 0.513.2 ± 0.5
    iNOS KO, high-fat23.4 ± 0.832.1 ± 0.7
Fasting glucose (mmol/l)
    WT, standard6.4 ± 0.66.5 ± 0.4
    WT, high-fat12.0 ± 0.3*11.2 ± 0.6*
    iNOS KO, standard7.0 ± 0.26.1 ± 0.2
    iNOS KO, high-fat8.5 ± 0.57.7 ± 0.2
Fasting insulin (pg/ml)
    WT, standard0.5 ± 0.10.3 ± 0.1
    WT, high-fat1.0 ± 0.2*3.5 ± 0.5*
    iNOS KO, standard0.5 ± 0.10.4 ± 0.1
    iNOS KO, high-fat0.6 ± 0.10.5 ± 0.1
HOMA (unit)
    WT, standard0.15 ± 0.010.15 ± 0.2
    WT, high-fat0.55 ± 0.1*2.03 ± 0.2*
    iNOS KO, standard0.14 ± 0.010.13 ± 0.01
    iNOS KO, high-fat0.18 ± 0.010.22 ± 0.02
Free fatty acids (mmol/l)
    WT, standard0.42 ± 0.1
    WT, high-fat1.41 ± 0.1*
    iNOS KO, standard0.35 ± 0.1
    iNOS KO, high-fat0.56 ± 0.1
Decline in glucose in response to insulin (%)
    WT, standard70.2 ± 1.965.6 ± 1.2
    WT, high-fat54.9 ± 0.6*48.5 ± 1.7*
    iNOS KO, standard68.1 ± 2.666.2 ± 1.7
    iNOS KO, high-fat68.0 ± 1.368.5 ± 1.2
Systolic blood pressure (mmHg)
    WT, standard117.6 ± 0.3118.6 ± 0.5
    WT, high-fat122.7 ± 0.3*124.8 ± 0.7*
    iNOS KO, standard113.9 ± 0.4114.9 ± 0.5
    iNOS KO, high-fat122.5 ± 0.5124.0 ± 0.7
  • Data are means ± SE.

  • *

    * P < 0.05 for standard vs. high-fat diet;

  • P < 0.05 for WT vs. iNOS KO mice on a high-fat diet.