TABLE 2

Cytokines produced during age-matched or unmatched cocultures using CD4+CD25+ and CD4+CD25 T-cells

CD25+ 6 weeks + CD25 6 weeksCD25+ diabetic + CD25 6 weeksCD25+ diabetic + CD25 diabeticCD25+ 6 weeks + CD25 diabetic
IL-4 (pg/ml)18.120.529.826.7
IL-10 (pg/ml)251.1146.2190.9337.4
IFN-γ (pg/ml)434.2558.82,184.71,815.9
TGF-β (pg/ml)121.360.669.6120.7
  • CD4+CD25+ or CD4+CD25 T-cells from 6-week-old or diabetic mice were stimulated with CD3 antibody and APCs. “Age-matched” or “crossed” cocultures were performed, and supernatants were recovered 24, 48, and 72 h later.