Oligonucleotide sequences for primer/probe sets used Taqman analysis
| Gene | Accession number | Forward primer | Probe | Reverse primer |
|---|---|---|---|---|
| ND 1 | CCCTAAAACCCGCCACATCT | CCATCACCCTCTACATCACCGCCC | GAGCGATGGTGAGAGCTAAGGT | |
| ND 4 | CCATTCTCCTCCTATCCCTCAAC | CCGACATCATTACCGGGTTTTCCTCTTG | CACAATCTGATGTTTTGGTTAAACTATATTT | |
| LPL | NM_000237 | CGAGTCGTCTTTCTCCTGATGAT | ACATTCACCAGAGGGTC | TTCTGGATTCCAATGCTTCGA |
| PGC-1α | NM_013261 | CACCAAACCCACAGAGAACA | CGCAGTCACAACACTTACAAGCCAAAC | GGGTCATTTGGTGACTCTGG |
| PGC-1β | NM_133263 | CAGCCACTCGAAGGAACTTCA | CTGAACACGGCCCTCTGCTCTCACA | CGGATGCTTGGCGTTCTG |
| mtTFA | NM_003201 | CCCAGATGCAAAAACTACAGAACTAA | TCCAACGCTGGGCAATTCTTCTA | TCCGCCCTATAAGCATCTTGA |
| Cyt C | NM_001916 | TGGCCCCTCCCATCTACAC | AGAGTTTGACGATGGCACCCCAGCTA | ATCCTTGGCTATCTGGGACATG |
| NRF-1 | NM_005011 | CGTTGCCCAAGTGAATTATTCTG | TTGTTCCACCTCTCCATCAGCCA | CCCTGTAACGTGGCCCAAT |
| PPAR-α | NM_005036 | GCTTTGGCTTTACGGAATACCA | AGCCATCTGAGCCAGGACAGCTTCCTAA | TGAAAGCGTGTCCGTGATGA |
| MCAD | AF251043 | TGCCCTGGAAAGGAAAACTTT | TGTAGAGCACCAAGCAATATCATTTATG | GTTCAACTTTCATTGCCATTTCAG |
| CPT-1 | D87812 | CCAGAGCAGCACCCCAAT | CATCTGCTACAGGGCCAAAGCCACCT | CTGCAATCATGTAGGAAACTCCATAG |
| MLYCD | NM_012213 | TGGCACTGACTGGTGACATCTC | AACATCCAGGCAATCGTGAAGGAACATC | TGTTCTTCTCTTCTGTTTCTGATGGA |
| UCP-1 | NM_021833 | ACGACACGGTCCAGGAGTTC | TCACCGCAGGGAAAGAAACAGCACC | ACCAGCTAAAATCTTGCTTCCTAAAC |
| TIF-2 | NM_006540 | AGTGACCTCCGTGCCTACGT | AGGATCATTAACCTGCTCGGGACCCAT | CAGGTTGCCTCCCCTCAGA |
| SRC-1 | U40396 | GCAGATGCCAGGAATGAACA | TGCCCTGAGCAGATAAATGATCCC | TTGCAGTAGAGGCCTGTGTGTCT |
| ADRB3 | NM_000025 | CGCAGCCCAGGCTTT | AGCCCCGTCGAGCCGTTG | CTTCTTGTCCTTCAGGCCTAAG |
| Cyclophilin B | XM_042251 | GGAGATGGCACAGGAGGAAA | CATCTACGGTGAGCGCTTCCCCG | CGTAGTGCTTCAGTTTGAAGTTCTCA |
ND, NADH dehydrogenase subunit 1; ND 4, NADH dehydrogenase subunit 4; LPL, liporotein lipase; Cyt C, cytochrome C; TIF-2, nuclear receptor coactivator-2; SRC-1, steroid receptor coactivator-1.