TABLE 4

Quantitative morphometric analysis of the pancreatic islet infiltration stages of IDDM rats

Pancreatic islet infiltration stages
Stage 0
Stage 1
Stage 2
Stage 3
Stage 4
mm2 (%)
    β-Cell area16.6 ± 0.2100 ± 212.0 ± 0.175 ± 37.5 ± 0.3*47 ± 54.2 ± 0.2*25 ± 300
    Infiltrated islet area18.6 ± 0.2100 ± 316.5 ± 0.591 ± 420.6 ± 0.6114 ± 521.0 ± 0.4116 ± 600
Cells/mm2 (%)
    Total immune cells1 ± 110098 ± 8100188 ± 12100301 ± 1710000
    ED1 macrophages1 ± 110067 ± 156987 ± 224678 ± 232600
    CD 4+ T-cells0012 ± 71223 ± 121227 ± 5900
    CD 8+ T-cells0013 ± 51361 ± 2033183 ± 486100
    NK-cells002 ± 1217 ± 397 ± 2200
    B-cells004 ± 14006 ± 1200
  • Data are means ± SE. β-Cell area and immune cell infiltration were determined by quantitative morphometry in pancreata from IDDM rats (45–65 days of age). Islet infiltration was graded on a score of 0–4 as follows: stage 0, no immune cell infiltration of the islets (control); stage 1, immune cell infiltration restricted to the periphery of the islets; stage 2, weak invasive immune cell infiltration of the islets; stage 3, large numbers of immune cells within most islets; and stage 4, complete β-cell loss without infiltration (end-stage islets). A mean insulitis score was calculated from 10 islets per individual pancreas. The numbers of analyzed rats were 12 in stage 0, 5 in stage 1, 9 in stage 2, 7 in stage 3, and 12 in stage 4.

  • *

    * P < 0.01 compared with stage 0 of islet infiltration

  • P < 0.05

  • P < 0.01 compared with stage 1 of islet infiltration (all by ANOVA/Dunnett test).