TABLE 1

Candidate A2-restricted epitopes

No.PositionSequence (human PI)Mouse PI-1 (PI-2)Binding score
HLA-A2 affinityTAP affinityProteasome cleavages*
SYFPEITHIBIMAS
1B9-B18SHLVEALYLVP(-)---------190.40.86.73
2B10-B18HLVEALYLV---------2722.32.5423
3B18-B27VCGERGFFYT---------20.527917
4C6-C14DLQVGQVEL-P--E(A)-L--251.637049514
5C22-C30SLQPLALEGD--T----V18122>1,0001
6C27-C35ALEGSLQKR---VAR(Q)---191544433
7C31-A5SLQKRGIVEQAR(Q)------D-201854827
8A1-A10GIVEQCCTSI---D------216.06.3121
9A8-A16TSICSLYQL------170.31.24.80
10A12-A20SLYQLENYC------1587.460.90
Reference peptide (FLPSDFFPSV/ RRYNASTEL)242,31011NA
  • Sequence identity between human and murine proinsulin is indicated by a dash. The binding score indicates a rank ≤11 for the indicated algorithm. HLA-A2 and TAP affinities are expressed as relative affinities (i.e., IC50 of test peptide/IC50 of reporter peptide).

  • *

    * Number of proteasome cleavages after COOH-terminal. NA, not applicable; PI, proinsulin.