TABLE 2

Comparison between microarrays and RTqPCR ratios of SVF- and adipocyte-overexpressed genes

GeneRatio microarrayQ value <5%Adipocyte-to-SVF ratio (RTqPCR)
SVF
    IL1Ra0.240.0140.41*
    MCP-10.360.0140.28*
    TNFα0.230.0140.10*
    A2M0.380.0140.49*
    CD680.140.0140.13*
    ITGAL0.840.370.11*
    CSFR10.540.0140.09*
    MMP90.270.0140.08*
    SDF1/CXCL120.620.0140.19*
    HF10.540.730.20*
    SERPA30.810.0880.21*
    MIP-3β0.760.0910.30*
Adipocyte
    FGF132.170.0146.10*
    PTGER33.560.0144.70*
    CES14.060.01411.7*
  • The following genes overexpressed in the SVF were validated by RTqPCR: IL1Ra, MCP-1, TNFα, A2M, CD68, ITGAL, CSF1R, MMP9, SDF1/CXCL12, HF1, SERPA3, MIP-3β. FGF13, PTGER3, and CES1, genes were preferentially expressed in isolated mature adipocytes, confirming the microarray data ratios. Q values are for differences in gene expression between adipocytes and SVF after application of SAM procedure to microarray ratios. Adjustment was performed for testing multiple hypotheses. Corresponding adipocyte/SVF mean ratios obtained by RTqPCR are given. Normalization was performed with 18S mRNA for RTqPCR experiments.

  • *

    * P < 0.05, Wilcoxon nonparametric paired test.