Comparison between microarrays and RTqPCR ratios of SVF- and adipocyte-overexpressed genes

GeneRatio microarrayQ value <5%Adipocyte-to-SVF ratio (RTqPCR)
  • The following genes overexpressed in the SVF were validated by RTqPCR: IL1Ra, MCP-1, TNFα, A2M, CD68, ITGAL, CSF1R, MMP9, SDF1/CXCL12, HF1, SERPA3, MIP-3β. FGF13, PTGER3, and CES1, genes were preferentially expressed in isolated mature adipocytes, confirming the microarray data ratios. Q values are for differences in gene expression between adipocytes and SVF after application of SAM procedure to microarray ratios. Adjustment was performed for testing multiple hypotheses. Corresponding adipocyte/SVF mean ratios obtained by RTqPCR are given. Normalization was performed with 18S mRNA for RTqPCR experiments.

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    * P < 0.05, Wilcoxon nonparametric paired test.