TABLE 3

Comparison of gene expression in isolated adipocytes and adipose tissue-derived macrophages by RTqPCR

GeneAdipocytesMacrophages
MCP10.047 ± 0.0110.28 ± 0.1**
A2M0.016 ± 0.0030.204 ± 0.1**
TNFα0.024 ± 0.0060.356 ± 0.062***
CD680.027 ± 0.0051.55 ± 0.24**
IL100.001 ± 0.00020.102 ± 0.038***
IL1Ra0.005 ± 0.00361.06 ± 0.3***
HIF-1α0.037 ± 0.00250.549 ± 0.053***
  • Data are mean RTqPCR ratio ± SE. Macrophages were isolated from adipose tissue SVF cells and gene expressions of MCP-1, A2M, TNFα, CD68, IL10, IL1Ra, and HIF-1α subunit were compared with those of isolated mature adipocytes. mRNA arbitrary units were normalized using 18S ribosomal RNA.