TABLE 1

Ca2+ transient characteristics

nΔF/F0TTP (ms)D1/2(ms)τ (ms)
Wild-type mice
    Control333.6 ± 0.327.9 ± 3.8167 ± 1136.1 ± 2.9
    Insulin (60 nmol/l)154.6 ± 0.3*26.0 ± 2.7175 ± 837.1 ± 2.6
    + Wortmannin (0.5 μmol/l)63.7 ± 0.529.0 ± 2.3167 ± 537.7 ± 3.1
    IP3 (10 μmol/l)183.8 ± 0.542.0 ± 4.1*211 ± 12*39.6 ± 4.1
Ob/ob mice
    Control212.5 ± 0.323.1 ± 2.1189 ± 1046.8 ± 2.9
    Insulin (60 nmol/l)182.7 ± 0.450.3 ± 6.8*240 ± 13*39.1 ± 3.6
    + Wortmannin (0.5 μmol/l)52.4 ± 1.130.1 ± 7.4194 ± 947.5 ± 3.9
    IP3 (10 μmol/l)133.2 ± 0.261.2 ± 9.6*284 ± 27*40.2 ± 4.1
  • Data are means ± SE.

  • *

    * P < 0.05 vs. the basal condition within each group (wild-type or ob/ob);

  • P < 0.05, ob/ob vs. wild-type when studied under the same conditions. ΔF/F0, peak amplitude; TTP, time to peak; D1/2, half-width; τ, decay time constant.