TABLE 1

TUNEL and Ki-67 labeling of β-cell nuclei

IL-1β (ng/ml)TUNEL
Ki-67
Number of cells countedNumber of TUNEL-labeled nucleiPercent of TUNEL-labeled nucleiNumber of cells countedNumber of Ki-67–labeled nucleiPercent of Ki-67–labeled nuclei
014,800570.385121,300390.1831
0.018,500150.176510,100200.1980
0.0216,400500.304918,600630.3387
0.216,900790.467518,000390.2167
18,100300.370410,500170.1619
215,5001320.851622,100300.1357
516,9002151.272219,800220.1111
  • Human islets were cultured on extracellular matrix–coated dishes for 4 days at 5.5 mmol/l glucose with increasing concentrations of IL-1β and double stained for β-cell apoptosis with the TUNEL assay and for proliferation with anti–Ki-67. Islets were isolated from four organ donors.