TABLE 1

In silico analysis of INS transcripts derived from EST libraries

EST library*Total number of available ESTsHphI allele/minisatelliteIsoform 4 (%)Isoform 6 (%)
HR85 islet192A/class I4715 (24)
Human insulinoma3,026T/class III82525 (3)§
  • *

    * Genbank accession numbers for each database entry and corresponding sequences are available in online appendix Table 2. The proportion of informative ESTs was similar in both libraries (∼30%).

  • INS haplotypes and isoform structure were determined using Python scripts (www.python.org) available in online appendix Table 3.

  • Of 15 ESTs, 10 were informative for the IVS1+5insTTGC polymorphism (INS-69+/−), and all of them had the deletion allele.

  • §

    § Of 25 ESTs, 10 were informative for the IVS1+5insTTGC polymorphism, and all of them had the insertion allele, suggesting that the insulinoma donor was of African origin.