TABLE 1

Ad-MafB activates endogenous glucagon gene transcription in βTC-3 cells and not NIH3T3

Ad-GFPAd-MafB
10×50×
βTC-3
    MafB1.0045.521,230.5718,260.62
    Glucagon1.002.023.487.83
    Insulin I1.001.291.371.30
    Insulin II1.001.091.041.00
    pdx11.001.490.830.81
    γ-Actin1.001.170.780.88
NIH3T3
    MafB1.0027.61143.991,486.43
    GlucagonNDNDNDND
    Insulin INDNDNDND
    pdx1NDNDNDND
    γ-Actin1.000.980.850.96
  • Data are expressed as the normalized fold difference relative to GFP alone (defined as 1). MafB and glucagon mRNA levels in βTC-3 and NIH 3T3 cells infected with Ad-GFP (MOI 100) or Ad-MafB (1× = MOI 2; 10× = MOI 20; 50× = MOI 100). All of the expression data were derived by real-time PCR analysis. A representative experiment is shown (n = 3). ND, not detected.