TABLE 1

AT1R expression in control and db/db islets using the comparative threshold cycle method

GeneCT*ΔCTΔΔCTExpression relative to control§
Control
    AT1R26.52 ± 0.626.62 ± 0.48
    18S19.79 ± 0.53
Diabetes
    AT1R24.54 ± 1.646.15 ± 0.76−0.47 ± 0.773.06
    18S18.35 ± 0.95
  • Data are means ± SE.

  • *

    * Sample average of threshold cycle (CT) data.

  • The ΔCT value was calculated by the subtraction of the 18S rRNA CT from each sample CT.

  • The ΔΔCT value was calculated by subtraction of the control ΔCT from each transplanted sample ΔCT.

  • §

    § Calculated using the equation 2−ΔΔCT.