TABLE 1

Primary antibodies for NF-κB protein detection by Western blot

ProteinSpeciesAntibody dilutionMouse kDa band
Canonical NF-κB proteins
    RelA/p65
Santa Cruz sc372Goat1:50060
Novus Biological ab7970Rabbit1:1,000
Rockland 100-4165Rabbit1:4,000
    p50
Cell Signaling 3035Rabbit1:50050
Calbiochem PC136Rabbit1:500
Santa Cruz sc114Rabbit1:1,000
    IκB-α35
Santa Cruz sc371Rabbit1:500
Rockland 100-4167CRabbit1:4,000
    IκB-β
Santa Cruz sc945Rabbit1:1,00038
Rockland 100-4186Rabbit1:4,000
    IKK-β
Santa Cruz sc8014Mouse1:1,00084–85
Calbiochem OP134Mouse1:500
Noncanonical NF-κB proteins
    RelB
Cell Signaling 4954Rabbit1:1,00060
Santa Cruz sc 226*Rabbit1:500
    p52
Upstate 05361Rabbit1:50044–45
Santa Cruz sc298Rabbit1:500
    NIK
Biovision1:1,000103
Cell Signaling 4994Rabbit1:1,000
    IKK-α
Calbiochem OP133Mouse1:50084–85
Santa Cruz sc76071:1,000
  • *

    * RelB antibody (sc226) was obtained from lot number L0204. Subsequent lots of this antibody are less immunoreactive for RelB in mouse renal cortical extracts.