TABLE 4

Silencing of NFAT5 reduces transcription activity of AKR1B1 in transfected HEK 293 cells

Z-2/C-106
Z/C-106
Normal glucoseHigh glucoseNormal glucoseHigh glucose
No-siRNA87.4 ± 14.9 (6)119.4 ± 39.0 (6)*35.1 ± 8.3 (7)51.4 ± 8.9 (7)
569R81.4 ± 1.9 (6)78.4 ± 10.3 (6)33.1 ± 8.8 (4)38.8 ± 9.2 (4)§
Inv569R105.5 ± 10.2 (5)122.4 ± 8.1 (5)44.2 ± 7.9 (5)59.4 ± 9.1 (5)
  • Data are means ± SE (n experiments performed). Results are the relative activity of transcription and were defined as luciferase activity normalized by the activity of the internal control. Duplicated wells were performed for each transfection. Transfected cells were culture under normal and high-glucose conditions (31 mmol/l d-glucose).

  • *

    * P = 0.028 vs. normal glucose;

  • P = 0.018 vs. normal glucose;

  • P = 0.04 vs. high glucose with No-siRNA and inv569R;

  • §

    § P = 0.04 vs. high glucose with No-siRNA and Inv569R.