TABLE 4

Ratio of gene expression in visceral fat from type 2 diabetic versus nonobese women

GeneType 2 diabetic/nonobese
P
Array*PCR
Electron transport chain genes unchanged on microarrays
    NDUFB20.930.970.44
    SDHC1.081.110.21
    COX150.900.970.39
    NDUFC11.040.960.38
    SLC25A40.901.020.42
Downregulated on microarrays
    COX5B0.710.790.06
    NDUFB40.780.750.02
    DAP130.630.900.19
    ATP5C10.770.730.04
    ATP5F10.700.860.10
Transcription factors and coregulators implicated in regulation of electron transport gene expression
    NRF10.91
    PGC1αAbsent0.840.26
    PGC1βNo probes1.740.0006
    TNF-αAbsent1.20NS
    TNF-α§Absent2.780.04
    ESRRA§1.091.000.49
    GABPA§0.941.370.003
    GABPB§Absent1.270.007
  • *

    * Array data are mean fold change of the probesets representing each genes that are present in more than six samples in at least one group;

  • gene expression was quantified in dataset 1 with real-time quantitative PCR and normalized to 18S, LRP10, and GAPDH. Type 2 diabetes (n = 8), nonobese control subjects (n = 6);

  • not quantified by real-time quantitative PCR. Represented by several probesets on the arrays. Expression was not affected by type 2 diabetes.

  • §

    § TNF-α, ESRRA, GABPA, and GABPB were quantified in dataset 2 and normalized to 18S. n = 10 type 2 diabetes, n = 10 nonobese control subjects. Real-time quantitative PCR results were compared with unpaired t test (one sided).