Mouse strainnEarly-onset diabetesLate-onset diabetesTotal diabetes
  • Mice were monitored weekly via random blood glucose levels immediately prior to and after injection with low-dose STZ. On the 4th week after STZ administration, 4-h fasting blood glucose was obtained prior to injection with 50% dextrose solution. Early-onset diabetes was defined as two consecutive blood glucose levels >350 mg/dl at week 1 and two post–STZ administration. Those mice were removed from the protocol and did not undergo a glucose challenge. Late-onset diabetes was defined as 4-h fasting blood glucose >240 mg/dl at the end of the 4-week period. All early-onset diabetic mice were males weighing 25–30 g, and thus they received the largest dosages of STZ administration. This effect was felt to be a dose effect of the STZ administration. In the STZ-administered CFTR−/− group, there were five males and five females. The male CFTR−/− mice weights were ∼25 g, whereas the C57BL/6J mice weighed 25–30 g. Female mice of both strains weighed ∼20 g. In the CFTR−/− group, four of five CFTR−/− males developed fasting hyperglycemia, and two of five females developed fasting hyperglycemia.