TABLE 1

Effects of leptin treatment on ob/ob mice

ob/ob
ControlVehicleLeptinInsulinLeptin + insulin
Change in weight (g)0 ± 0.3−6.4 ± 1.4*0.6 ± 0.3−4.3 ± 0.3
Food intake (g · day−1 · mouse−1)6.2 ± 0.411.3 ± 0.71.2 ± 0.39.2 ± 0.4*2.1 ± 0.4
Fasting blood glucose (mg/dl)143 ± 9268 ± 72165 ± 21162 ± 2892 ± 11
Fasting insulin (ng/ml)1.1 ± 0.47.7 ± 0.41.1 ± 0.217.6 ± 2.4*7.4 ± 1.1
Leptin (ng/ml)3.2 ± 0.5027.4 ± 4.5*08.4 ± 1.4
  • Data are means ± SE (n = 4–6). Vehicle-treated ob/ob mice and their untreated lean control littermates (Control) were compared with ob/ob mice treated with leptin (24 μg/day), insulin, or both for 4 days. The weight change and food intake were measured over the 4 days and the last 24 h, respectively. After 2 days of treatment, mice were fasted for 4 h and insulin and blood glucose were measured. Leptin was measured in the nonfasted state at the end of the experiment.

  • *

    * P < 0.05,

  • P < 0.005 vs. untreated ob/ob;

  • P < 0.05 vs. treatment with leptin alone.