TABLE 2

Temporal phosphorylation dynamics of sites with at least 10-fold increase after 5-min insulin stimulation

ProteinPhosphorylation siten*0:5 min5:5 min15:5 min45:5 min
APSY61830.07 ± 0.0110.98 ± 0.090.82 ± 0.17
Cdc42bpbY164030.09 ± 0.0110.94 ± 0.060.80 ± 0.09
ERK1T202 Y20430.05 ± 0.0010.59 ± 0.080.37 ± 0.03
ERK2T185 Y18730.07 ± 0.0210.66 ± 0.030.42 ± 0.03
Gab1Y66030.07 ± 0.0210.61 ± 0.130.33 ± 0.06
Insulin receptorY1175 / Y1179 / Y1180 (1p)30.08 ± 0.0111.05 ± 0.040.82 ± 0.12
Y1175 / Y1179 / Y1180 (2p)30.03 ± 0.0110.99 ± 0.060.81 ± 0.08
T1344 / Y1345 Y1351 (2p)30.06 ± 0.0111.03 ± 0.110.85 ± 0.12
Y1345 / Y1351 (1p)10.0711.070.94
IRS-1Y117130.03 ± 0.0111.02 ± 0.070.77 ± 0.06
Munc18cY52130.07 ± 0.0111.11 ± 0.011.20 ± 0.14
SPRY4Y5330.07 ± 0.0210.87 ± 0.080.31 ± 0.01
  • Data are means ± SD.

  • *

    * Number of replicate analyses in which the site could be quantified.

  • Average values are given; for single values see online appendix.