TABLE 2

Citrate synthase activity and protein yield in IMF and SS skeletal muscle mitochondria at the end of semistarvation and after 7 days of refeeding

SemistarvationControlRestricted
Citrate synthase activity (μmol · min−1 · g−1 tissue)
    Homogenate20.0 ± 0.6516.7 ± 0.73*
    IMF6.46 ± 0.504.77 ± 0.46*
    SS2.39 ± 0.301.06 ± 0.16*
Citrate synthase specific activity (μmol · min−1 · mg−1 protein)
    IMF3.20 ± 0.103.50 ± 0.10
    SS4.20 ± 0.103.80 ± 0.20
Protein yield (mg/g tissue)
    IMF1.99 ± 0.191.32 ± 0.18*
    SS0.57 ± 0.030.28 ± 0.04*
RefeedingControlRefed
Citrate synthase activity (μmol · min−1 · g−1 tissue)
    Homogenate16.2 ± 0.5613.1 ± 0.63*
    IMF4.54 ± 0.324.11 ± 0.37
    SS1.52 ± 0.121.20 ± 0.08*
Citrate synthase specific activity (μmol · min−1 · mg−1 protein)
    IMF2.95 ± 0.083.04 ± 0.10
    SS3.19 ± 0.063.34 ± 0.26
Protein yield (mg/g tissue)
    IMF1.56 ± 0.121.35 ± 0.11
    SS0.49 ± 0.050.34 ± 0.02*
  • Data are means ± SE of six different experiments.

  • *

    * P < 0.05 compared with controls.