TABLE 1

The HLA-A2–restricted epitope panel used in the ISL8Spot assay

SequenceSYFPEITHI scoreReference
PPI2–10ALWMRLLPL28Unpublished
PPI6–14RLLPLLALL31Unpublished
PIB10–18 (PPI34–42)HLVEALYLV27Hassainya et al. (21); Toma et al. (23); Pinkse et al. (24)
PIB18–27 (PPI42–51)VCGERGFFYT7Hassainya et al. (21); Toma et al. (23)
PIC20–28 (PPI76–84)SLQPLALEG18Hassainya et al. (21)
PIC29-A5 (PPI85–94)SLQKRGIVEQ20Hassainya et al. (21)
PIA1-A10 (PPI90–99)GIVEQCCTSI21Hassainya et al. (21)
PIA12–20 (PPI101–109)SLYQLENYC15Hassainya et al. (21)
GAD65114–123VMNILLQYVV21Panina-Bordignon et al. (29)
IGRP228–236LNIDLLWSV22Takaki et al. (20)
IGRP265–273VLFGLGFAI24Takaki et al. (20)
Flu MP58–66GILGFVFTL30Viral mix positive control
EBV BMLF1280–288GLCTLVAML28Viral mix positive control
CMV pp65495–503NLVPMVATV30Viral mix positive control
HIV gag77–85SLYNTVATL31Negative control
  • For PPI, amino acid (aa) numbering is given both with respect to the PI sequence alone (aa B1-A21) and to the complete PPI sequence (aa 1–110).