Effects of long-term culture in high glucose and lipids on insulin content and secretion
Culture condition (72 h) | Insulin content (ng/islet) | Insulin secretion (ng/islet per h) during 1-h incubation | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 mmol/l glucose (A) | 20 mmol/l glucose (B) | B–A | 20 mmol/l glucose + 0.1 mmol/l tolbutamide (C) | |||||||||||||||
Means | n | Means | n | Means | n | Means | n | Means | n | |||||||||
4.5 mmol/l glucose | 59 ± 6 | 4 | 0.28 ± 0.05 | 4 | 2.98 ± 0.30* | 4 | 2.70 ± 0.15 | 4 | ND | — | ||||||||
4.5 mmol/l glucose + 0.5 mmol/l oleate | 56 ± 9 | 4 | 0.55 ± 0.05† | 4 | 0.91 ± 0.14‡ | 4 | 0.36 ± 0.07§ | 4 | ND | — | ||||||||
4.5 mmol/l glucose + 0.5 mmol/l palmitate | 70 ± 12 | 4 | 0.51 ± 0.02† | 4 | 0.80 ± 0.09‡ | 4 | 0.29 ± 0.05§ | 4 | ND | — | ||||||||
15 mmol/l glucose | 198 ± 38 | 4† | 0.49 ± 0.03 | 8† | 2.36 ± 0.26* | 8 | 1.89 ± 0.09§ | 8 | 3.13 ± 0.29‖ | 8 | ||||||||
15 mmol/l glucose + oleate | 59 ± 4 | 4¶ | 0.50 ± 0.03 | 8 | 0.99 ± 0.07* | 8 | 0.49 ± 0.03# | 8 | 1.18 ± 0.16 | 8 | ||||||||
15 mmol/l glucose + palmitate | 48 ± 8 | 4¶ | 0.52 ± 0.03 | 8 | 1.12 ± 0.11* | 8 | 0.60 ± 0.04# | 8 | 1.22 ± 0.17 | 8 |
* P < 0.001 vs. 1 mmol/l glucose;
† P < 0.001 vs. line 1;
‡ P < 0.05 vs. 1 mmol/l glucose;
§ P < 0.001 vs. line 1 same column;
‖ P < 0.001 vs. line 4 same column;
¶ P < 0.001 vs. line 4 same column;
# P < 0.001 vs. line 4 same column. ND, not determined.