TABLE 1

Effects of long-term culture in high glucose and lipids on insulin content and secretion

Culture condition (72 h)Insulin content (ng/islet)
Insulin secretion (ng/islet per h) during 1-h incubation
1 mmol/l glucose (A)
20 mmol/l glucose (B)
B–A
20 mmol/l glucose + 0.1 mmol/l tolbutamide (C)
MeansnMeansnMeansnMeansnMeansn
4.5 mmol/l glucose59 ± 640.28 ± 0.0542.98 ± 0.30*42.70 ± 0.154ND—
4.5 mmol/l glucose + 0.5 mmol/l oleate56 ± 940.55 ± 0.05†40.91 ± 0.14‡40.36 ± 0.07§4ND—
4.5 mmol/l glucose + 0.5 mmol/l palmitate70 ± 1240.51 ± 0.02†40.80 ± 0.09‡40.29 ± 0.05§4ND—
15 mmol/l glucose198 ± 384†0.49 ± 0.038†2.36 ± 0.26*81.89 ± 0.09§83.13 ± 0.29‖8
15 mmol/l glucose + oleate59 ± 440.50 ± 0.0380.99 ± 0.07*80.49 ± 0.03#81.18 ± 0.168
15 mmol/l glucose + palmitate48 ± 840.52 ± 0.0381.12 ± 0.11*80.60 ± 0.04#81.22 ± 0.178
  • *

    * P < 0.001 vs. 1 mmol/l glucose;

  • †

    † P < 0.001 vs. line 1;

  • ‡

    ‡ P < 0.05 vs. 1 mmol/l glucose;

  • §

    § P < 0.001 vs. line 1 same column;

  • ‖

    ‖ P < 0.001 vs. line 4 same column;

  • P < 0.001 vs. line 4 same column;

  • #

    # P < 0.001 vs. line 4 same column. ND, not determined.