TABLE 2

Fold changes in islet mRNA levels after long-term incubation with glucose and lipids

Culture conditionSnap25a
Chgb
Cpt1
GLUT2
Ins1
Syt4l/Slp4
MeansnMeansnMeansnMeansnMeansnMeansn
15 mmol/l glucose1.8 ± 0.24*44.6 ± 0.88*40.91 ± 0.1458.7 ± 1.62*420.5 ± 5.05*54.0 ± 0.6*4
0.5 mmol/l palmitate + 15 mmol/l glucose1.0 ± 0.0851.4 ± 0.14*52.5 ± 0.1960.68 ± 0.0560.77 ± 0.0450.82 ± 0.04*4
0.5 mmol/l oleate + 15 mmol/l glucose0.88 ± 0.1241.5 ± 0.06*43.2 ± 0.3240.49 ± 0.14*60.79 ± 0.06*60.93 ± 0.204
0.5 palmitate + 4.5 mmol/l glucose0.93 ± 0.0661.1 ± 0.162.6 ± 0.1150.87 ± 0.1451.8 ± 0.26*51.1 ± 0.056
0.5 mmol/l oleate + 4.5 mmol/l glucose1.0 ± 0.0961.2 ± 0.1262.6 ± 0.2450.67 ± 0.251.6 ± 0.2141.1 ± 0.16
  • Data are the ratios of mRNA levels relative those measured at the respective glucose concentration. The effects of high glucose are normalized to expression in islets cultured at 4.5 mmol/l glucose.

  • *

    * P < 0.05,

  • P < 0.01, and

  • P < 0.001 vs. FFA-free medium containing 4.5 or 15 mmol/l glucose. Chgb, granule protein chromogranin B; Cpt1, carnitin palmitoyl transferase-1; Ins1, insulin gene 1; Snap25a, synaptosomal-associated protein 25, isoform a; Syt4l/Slp4 synaptotagmin-like protein 4.