Primers and product size for real-time RT-PCR
| Target genes | GenBank accession no. | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|
| Gapdh | NM_017008.3 | 5′- GAGCTGAATGGGAAGCTCAC-3′ | 5′-AAAGGTGGAGGAGTGGGAGT-3′ | 216 |
| β-actin | NM_031144.2 | 5′-AGCCATGTACGTAGCCATCC-3′ | 5′- CTCTCAGCTGTGGTGGTGAA−3′ | 228 |
| Vegf-a | NM_031836.1 | 5′-AGAAACCCAATGAAGTGGTG-3′ | 5′- ACTCCAGGGCTTCATCATTG-3′ | 177 |
| Flt-1 | NM_019306.1 | 5′- TCCCTCAGCCTACCATCAAG-3′ | 5′- GAGAGTCAGCCACCACCAAT-3′ | 207 |
| Flk-1 | NM_013062.1 | 5′-ACAGCATCACCAGCAGTCAG-3′ | 5′- CCAAGAACTCCATGCCCTTA-3′ | 168 |
The pairs of primers were designed according to rat gene sequences published in GenBank.