TABLE 2

Comparison of angiogenic effects of duct cell–purified and endocrine cell–purified fractions in the CAM assay

InoculateNumber of blood vessels per implantP
Duct cell purified
    106 cells
        0.1037.0 ± 0.0NS
        0.2542.5 ± 3.5≤0.05
        0.5041.0 ± 1.4≤0.01
Endocrine cell purified
    106 cells
        0.1014.0 ± 15.6
        0.2518.0 ± 8.7
        0.5024.0 ± 1.4
Concentration (μg/ml)
    BSA
        1,0009.3 ± 2.7
    Recombinant human VEGF
        10027.3 ± 7.6≤0.001*
    Recombinant human IL-8
        10023.2 ± 5.9≤0.001*
  • Data are the average of three implants per condition ± SE (of two to five independent experiments). Islet preparations were dissociated to single cells and fluorescence-activated cell sorted into duct cell–purified and endocrine cell–purified preparations before inoculation on CAMs at the indicated cell numbers. At day 13, the region around the implant was photographed and the number of vessels was counted. P vs. endocrine cell–purified preparation.

  • *

    * P vs. negative control (1,000 μg/ml; 0.1% BSA).