TABLE 2

Gene expression of proatherogenic mediators in the aorta

ParametersControl apoE−/−Diabetic apoE−/−Control RAGE−/−/apoE−/−Diabetic RAGE−/−/apoE−/−
n12101414
Inflammatory mediators
    p651.0 ± 0.12.6 ± 0.5*1.0 ± 0.11.5 ± 0.2*
    VCAM-11.0 ± 0.24.2 ± 0.6*1.7 ± 0.2*1.6 ± 0.4
    MCP-11.0 ± 0.26.2 ± 1.3*1.7 ± 0.4*2.6 ± 0.7*
    Tissue factor1.0 ± 0.22.9 ± 0.7*0.6 ± 0.31.7 ± 0.4
NADPH oxidase and RAS
    p47phox1.0 ± 0.25.1 ± 0.7*1.0 ± 0.21.8 ± 0.3*
    gp91phox1.0 ± 0.33.54 ± 0.5*1.22 ± 0.32.1 ± 0.3*
    rac11.0 ± 0.11.5 ± 0.2*1.4 ± 0.11.3 ± 0.2
    AT1a1.0 ± 0.25.3 ± 1.3*1.2 ± 0.31.4 ± 0.3
AGE receptors
    RAGE1.0 ± 0.14.4 ± 1.1*00
    AGE-R11.0 ± 0.12.4 ± 0.3*1.1 ± 0.11.4 ± 0.2
    AGE-R21.0 ± 0.12.1 ± 0.4*1.2 ± 0.11.7 ± 0.2
    AGE-R31.0 ± 0.34.6 ± 1.1*0.9 ± 0.11.8 ± 0.3
    CD361.0 ± 0.52.1 ± 0.4*0.8 ± 0.11.0 ± 0.2
Indicators of matrix accumulation and degradation
    Collagen I1.0 ± 0.14.1 ± 1.1*1.2 ± 0.13.1 ± 0.6*
    Collagen III1.0 ± 0.28.9 ± 2.9*1.7 ± 0.2*2.8 ± 0.6*
    Collagen IV (αI)1.0 ± 0.12.9 ± 0.7*1.6 ± 0.2*2.7 ± 0.6*
    Collagen IV (αIII)1.0 ± 0.13.7 ± 1.2*3.0 ± 0.7*2.3 ± 0.4*
    MMP-21.0 ± 0.23.0 ± 0.6*1.2 ± 0.32.1 ± 0.6
    MMP-91.0 ± 0.21.9 ± 0.4*1.1 ± 0.30.8 ± 0.2
  • Data are means ± SE.

  • *

    * P < 0.05 vs. control apoE−/− group;

  • P < 0.05 vs. diabetic apoE−/− group;

  • P < 0.05 vs. control RAGE−/−/apoE−/−.