TABLE 1

Steady-state kinetic parameters for the reduction of AGE precursors by AKRs

SubstratesGenbank accession numberProteinsKm (μM)kcat (min−1)kcat/Km (min−1/μM−1)
GlyoxalNM_021473AKR1A43,229 ± 90445.8 ± 4.20.014
NM_001628AKR1B1350 ± 2029.5 ± 2.020.082
NM_009658AKR1B3333 ± 6212.1 ± 0.360.036
NM_009731AKR1B77,450 ± 1,5005.6 ± 0.350.001
NM_008012AKR1B8635 ± 8120 ± 0.520.031
NM_020299AKR1B107,800 ± 3598.87 ± 0.630.002
NM_030611AKR1C3NDA
NM_013778AKR1C612,073 ± 1,03112.8 ± 0.670.001
NM_134066AKR1C18NDA
NM_025337AKR7A55,931 ± 1,39312.87 ± 0.270.002
MethylglyoxalAKR1A4874 ± 91483 ± 0.270.55
AKR1B122 ± 237.2 ± 2.211.698
AKR1B335 ± 419.5 ± 2.30.56
AKR1B71,996 ± 2748.9 ± 0.310.004
AKR1B8184 ± 1518.2 ± 1.210.098
AKR1B101,250 ± 8926.82 ± 50.389
AKR1C3NDA
AKR1C6139 ± 357.65 ± 0.70.054
AKR1C18NDA
AKR7A59,075 ± 1,25022 ± 0.70.002
FurfuralAKR1A45,918 ± 1,37917.4 ± 0.780.002
AKR1B1316 ± 3430.86 ± 1.910.097
AKR1B369 ± 1013.36 ± 0.931.91
AKR1B7NDA
AKR1B8622 ± 1002.6 ± 0.270.001
AKR1B101,617 ± 287178 ± 150.11
AKR1C3156 ± 353.09 ± 0.330.019
Hydroxymethyl FurfuralAKR1A46,428 ± 1,47924.05 ± 40.003
AKR1B1482 ± 9214.55 ± 20.03
AKR1B398 ± 154.15 ± 0.30.042
AKR1B7NDA
AKR1B81,197 ± 1177.82 ± 0.050.016
AKR1B101,117 ± 135100 ± 150.085
AKR1C31,939 ± 425167 ± 0.020.008
DeoxyglucosoneAKR1A42,067 ± 178777 ± 250.376
AKR1B1112 ± 538.9 ± 20.345
AKR1B389 ± 511.6 ± 10.13
AKR1B103,293 ± 11814.36 ± 1.90.004
AKR1B103,293 ± 11814.36 ± 1.90.004
  • Enzyme activity was measured in 0.1 mol/l potassium phosphate (pH 7.0) using the indicated substrates and 0.15 mmol/l NADPH at room temperature. Proteins were reduced with dithiothreitol before assay. NDA, no detectable activity.