TABLE 2

Glycation adduct residue contents of human fibronectin glycated by methylglyoxal and glucose (AGE)

Glycation adductMethylglyoxal-fibronectin control-1*Methylglyoxal-fibronectinAGE-fibronectin control-2AGE-fibronectin
Fructosyl-lysine0.213 ± 0.0340.215 ± 0.0880.094 ± 0.0220.610 ± 0.088
CML0.119 ± 0.0250.202 ± 0.0410.079 ± 0.0210.092 ± 0.020
CEL0.008 ± 0.0010.067 ± 0.0160.007 ± 0.0010.014 ± 0.005
G-H10.016 ± 0.0040.046 ± 0.007§0.005 ± 0.0020.008 ± 0.001
MG-H10.239 ± 0.01328.42 ± 0.970.176 ± 0.0060.198 ± 0.006
3DG-H0.217 ± 0.0680.819 ± 0.2150.420 ± 0.0660.727 ± 0.044§
Argpyrimidine<LOD0.793 ± 0.060<LOD<LOD
MOLD0.0021 ± 0.00010.0039 ± 0.00030.0015 ± 0.00010.0030 ± 0.0008
Pentosidine0.000015 ± 0.0000010.000129 ± 0.0000100.000007 ± 0.0000010.000012 ± 0.000001
MetSO1.05 ± 0.093.15 ± 0.160.40 ± 0.040.38 ± 0.02
  • Data are mol/mol fibronectin; means ± SD (n = 3).

  • *Fibronectin incubated under the conditions to control for glycation by methylglyoxal.

  • †Fibronectin incubated under the conditions to control for glycation by glucose. <LOD indicates argpyrimidine residue content is less than the limit of detection (<0.001 mol/mol fibronectin). Other adducts not detected were (mol/mol): GOLD and dityrosine <0.0006, DOLD <0.0002, and 3-NT <0.0001.

  • P < 0.05,

  • §P < 0.01,

  • P < 0.001; t test.