TABLE 3

Glycation adduct contents of laminin minimally modified by methylglyoxal and glucose (AGE)

Glycation adductMethylglyoxal- laminin control-1*Methylglyoxal- lamininAGE-laminin control-2AGE-laminin
FL0.777 ± 0.1000.698 ± 0.0471.11 ± 0.3521.84 ± 0.58
CML0.303 ± 0.0520.332 ± 0.0700.353 ± 0.0271.420 ± 0.094
CEL0.035 ± 0.0090.562 ± 0.0470.029 ± 0.0040.055 ± 0.010
G-H10.022 ± 0.0090.021 ± 0.0110.031 ± 0.0010.229 ± 0.060§
MG-H10.87 ± 0.0456.57 ± 1.781.44 ± 0.101.66 ± 0.16
3DG-H2.31 ± 0.291.34 ± 0.362.26 ± 0.165.97 ± 0.57§
Argpyrimidine<LOD6.82 ± 0.56<LOD<LOD
MOLD0.0030 ± 0.00110.0448 ± 0.00810.0018 ± 0.00060.0074 ± 0.0012§
Pentosidine0.000036 ± 0.0000020.000045 ± 0.0000010.000052 ± 0.0000020.000089 ± 0.000004
MetSO6.01 ± 0.235.91 ± 1.564.81 ± 0.883.89 ± 1.32
  • Data are mol/mol laminin; means ± SD (n = 3). <LOD indicates argpyrimidine residue content is less than the limit of detection (<0.002 mol/mol laminin). Other adducts not detected were (mol/mol): GOLD and dityrosine <0.0012, DOLD <0.0004, and 3-NT <0.0002.

  • *Laminin incubated under the conditions to control for glycation by methylglyoxal.

  • †Laminin incubated under the conditions to control for glycation by glucose.

  • P < 0.05,

  • §P < 0.01,

  • P < 0.001; t test.