TABLE 3

O-GlcNAc site-mapping and comparison of site-specific O-GlcNAc RORs between normal and diabetic states

Protein nameAccession no.O-GlcNAc peptidesPeptide scoreRatio D:NROR
Spectrin-β chain, erythrocyticP11277R.DVSSVELLMK.Y561.00.97
K.DLTSVLILQR.K431.11.0
K.LLTSQDVSYDEAR.N871.21.2
R.AQGLLSAGHPEGEQIIR.L541.00.97
R.LLSGEDVGQDEGATR.A601.00.97
Carbonic anhydrase 1P00915K.YSSLAEAASK.A580.200.40
K.ESISVSSEQLAQFR.S860.290.57
Hemoglobin subunit-βP68871R.FFESFGDLSTPDAVMGNPK.V531.81.8
K.VLGAFSDGLAHLDNLK.G751.21.2
K.GTFATLSELHCDK.L671.11.1
Band 3 anion transport proteinP02730K.ASTPGAAAQIQEVK.E860.670.66
K.HSHAGELEALGGVKPAVLTR.S530.700.69
K.IPPDSEATLVLVGR.A691.11.1
Ankyrin-1P16157K.LSTPPPLAEEEGLASR.I981.00.97
K.VVTDETSFVLVSDK.H521.51.5
R.ISEILLDHGAPIQAK.T491.11.1
R.DSGEGDTTSLR.L452.42.3
Spectrin-α chainP02549R.VSSQDYGR.D571.01.0
R.VILENIASHEPR.I421.01.0
R.LSESHPDATEDLQR.Q501.01.0
Peroxiredoxin-2P32119K.ASAVVDGAFK.E370.740.83
R.LSEDYGVLK.T510.820.92
Erythrocyte band 4.2P16452R.TQATFPISSLGDR.K370.900.86
GLUT1P11166R.TFDEIASGFR.Q591.11.3
Equilibrative nucleoside transporter 1Q99808K.DAQASAAPAAPLPER.N501.01.0
Protein 4.1P11171R.LTSTDTIPK.S431.01.1
Glutathione transferase ω-1P78417K.GSAPPGPVPEGSIR.I562.53.2
Proteasome subunit-α type 5P28066K.SSLIILK.Q541.31.4
CatalaseP04040R.LSQEDPDYGIR.D481.11.2
R.FSTVAGESGSADTVR.D782.13.4
α-SynucleinP37840K.TVEGAGSIAAATGFVK.K501.31.6
Aquaporin-1P29972R.SSDLTDR.V421.31.2
Hemoglobin subunit-αP69905K.FLASVSTVLTSK.Y651.21.2
R.MFLSFPTTK.T352.32.2
M.VLSPADK.T431.00.96
  • Data are means of three experiments. Peptide scores listed are the highest scores in three independent experiments. Ratio D:N, ratio between diabetic and normal samples. ROR, O-GlcNAc ROR. Underlined residues show site of modification.