TABLE 1

Blood glucose, serum insulin, plasma free fatty acids, and liver glycogen were analyzed in male PTP1B fl/fl control and Alb-Cre-PTP1B−/− mice (n = 6–18 mice/group) fed chow or HFD as indicated

Chow
HFD
PTP1B fl/fl (control)Alb-Cre-PTP1B−/−PTP1B fl/fl (control)Alb-Cre-PTP1B−/−
Blood glucose (mg/dl)
    Fasted 8 weeks55.4 ± 2.555.0 ± 1.775.0 ± 4.363.2 ± 5.7
    Fed 8 weeks111.1 ± 4.6111.6 ± 2.2111.6 ± 3.988.1 ± 2.3*
    Fasted 16 weeks70.8 ± 5.563.6 ± 4.972.4 ± 6.650.2 ± 7.3
    Fed 16 weeks103.7 ± 4.694.8 ± 3.3121.2 ± 5.5110.5 ± 7.3
Serum insulin (ng/ml)
    Fed 8 weeks0.64 ± 0.100.64 ± 0.081.30 ± 0.311.06 ± 0.15
    Fed 16 weeksNDND5.11 ± 1.112.35 ± 0.24*
    Pre-clamp (fasted)0.20 ± 0.050.13 ± 0.02NDND
    Clamp0.93 ± 0.180.72 ± 0.18
    GTT time 0 min0.30 ± 0.060.24 ± 0.06
    GTT time 15 min0.59 ± 0.080.73 ± 0.08
    GTT time 30 min0.54 ± 0.070.63 ± 0.04
Plasma free fatty acids (mmol/l)
    Fasted 16 weeksNDND0.885 ± 0.0750.942 ± 0.079
    Fed 16 weeks0.456 ± 0.0470.413 ± 0.0670.523 ± 0.0410.483 ± 0.065
Liver glycogen (μg/mg)
    FastedNDND2.16 ± 0.441.94 ± 0.63
    Fed19.92 ± 1.2220.91 ± 0.58
  • Data are means ± SE and were analyzed by two-tailed Student's t test.

  • *

    * P < 0.01,

  • P <0.05 for the indicated genotype compared with PTP1B fl/fl control littermates. ND, not determined.