TABLE 2

Genes regulated by high glucose or high glucose plus FOXO1 siRNA in RMECs

GeneUnique identificationHigh glucoseHigh glucose plus FOXO1 siRNA
MeanMedianMeanMedian
Endothelial cell inflammation/activation
    CCL2Rn.47722.21.70.40.3
    CCL5Rn.80192.12.10.10.1
    CXCL1Rn.109072.82.50.30.2
    ICAM1Rn0.122.32.00.40.4
    IL6Rn.98732.42.00.30.3
    IL7Rn.107932.31.70.30.2
    VCAM1Rn.112672.82.00.30.3
    ANXA5Rn.33181.61.60.60.6
    CCL20Rn.107222.41.60.30.3
    CPB2Rn.125721.91.20.50.6
    CSF2Rn.442851.41.20.30.2
    ICAM2Rn.1385193.01.10.20.2
    INOSRn.104001.91.30.40.5
    SEL-ERn.103591.71.00.40.3
    SEL-LRn.104611.81.10.30.3
    SEL-PRn.100122.01.20.30.3
    TGFB1Rn.401362.61.50.30.2
    TGFB2Rn.245392.31.50.30.2
    VWFRn.355611.71.10.40.3
    IL11Rn.472181.40.70.30.5
    IL15Rn.24902.01.10.20.2
    IL3Rn.106521.00.50.30.6
Endothelial cell apoptosis
    BAXRn.106682.52.00.50.5
    BCL2Rn.99962.52.10.40.4
    CASP3Rn.105622.92.40.50.5
    CASP6Rn.881604.14.20.30.3
    CASP8Rn.544742.01.90.50.5
    TNFRSF11BRn.97921.71.70.30.4
    TNFRSF10BRn.1055582.61.50.30.4
    BCL2A1Rn.197701.71.70.70.7
    BIRC3Rn.645781.81.70.70.7
    BIRC4Rn.912392.81.70.70.8
Angiogenesis
    ITGB1Rn.257332.62.00.30.3
    ANGPT1Rn.1202724.94.60.40.4
    FLT4Rn.810433.52.70.20.2
    VEGFARn.19233.02.80.40.3
    ITGA5Rn.1007962.81.10.40.4
    ITGB3Rn.171292.41.40.30.3
    KDRRn.888691.91.10.40.4
    MMP2Rn.64222.51.60.40.4
    MMP9Rn.102092.11.30.30.4
    TEKRn.91593.91.60.30.3
    COL18A1Rn.120302.21.30.50.5
    PLGRn.201781.71.10.50.6
  • Data are mean and median of three independent arrays for each time point. Boldface indicates that both the mean and median of the experimental group was 1.7- or 0.58-fold of the corresponding control (high glucose versus low glucose; high glucose plus FOXO1 siRNA versus high glucose plus scrambled siRNA). RMECs were incubated with or without high glucose (25 mmol/l) for 5 days. Some cells were transfected with FOXO1 siRNA or scrambled siRNA for 24 h prior to incubation in high glucose for 5 days. Total RNA was isolated and subjected to focused microarray analysis. The table lists genes differentially regulated by high glucose or FOXO1 siRNA in high-glucose–treated microvascular endothelial cells. High glucose: fold change in mRNA level in high-glucose–treated cells versus standard-glucose–treated cells. High glucose plus FOXO1 siRNA: fold change in mRNA level of each gene in cells transfected with FOXO1 siRNA plus high glucose versus scrambled siRNA plus high glucose.