TABLE 1

Effect of [2-13C]acetate infusions on physiological variables and substrates (concentrations and13C-enrichments) measured in arterial plasma of control and 3dRH rats under euglycemic and hypoglycemic conditions

Euglycemia
Hypoglycemia
Control
3dRH
Control
3dRH
BeforeAfterBeforeAfterBeforeAfterBeforeAfter
pH7.37 ± 0.027.40 ± 0.037.36 ± 0.017.35 ± 0.027.38 ± 0.017.43 ± 0.017.40 ± 0.017.47 ± 0.02*
Pco2(mmHg)38 ± 145 ± 241 ± 444 ± 435 ± 338 ± 235 ± 337 ± 4
Po2(mmHg)130 ± 7120 ± 5148 ± 5147 ± 9130 ± 10170 ± 15130 ± 10160 ± 15
Glucose
    mmol/l9.4 ± 0.712.5 ± 1.18.1 ± 0.0311 ± 0.32.3 ± 0.22.0 ± 0.22.3 ± 0.72.3 ± 0.7
    %E0n.d.0n.d.0n.d.0n.d.
BHB
    mmol/l0.3 ± 0.030.5 ± 0.10.3 ± 0.030.37 ± 0.071.2 ± 0.070.9 ± 0.061.2 ± 0.60.7 ± 0.4
    %E018 ± 0.7017 ± 5017.9 ± 1.303.3 ± 1.9
Lactate
    mmol/l1.3 ± 0.11.6 ± 0.31.0 ± 0.071.20 ± 0.021.0 ± 0.070.8 ± 0.071.0 ± 0.20.8 ± 0.4
    %E02.6 ± 0.402.5 ± 0.70n.d.0n.d.
Acetate
    mmol/l0.13 ± 0.019.6 ± 0.90.14 ± 0.0210.6 ± 1.50.13 ± 0.028.2 ± 20.1411 ± 5
    %E090 ± 0.7088 ± 1.3086 ± 2084 ± 6
  • The natural abundance of13C (1.1%) was subtracted from the percentage enrichments (%E).

  • *P< 0.05 and P< 0.01 for control vs. 3dRH;

  • P< 0.05 and P< 0.01 for euglycemia vs. hypoglycemia. n.d., non-detectable.