TABLE 2

Brain extract metabolite concentrations (μmol/g) and13C-percentage enrichments (%E) at the end of the [2-13C]acetate infusion

Euglycemia
Hypoglycemia
Control3dRHControl3dRH
Acetate
    Concentration (μmol/g)1.5 ± 0.21.2 ± 0.31.2 ± 0.10.8 ± 0.2
    C2 (%E)90 ± 0.788 ± 1.486 ± 284 ± 2
Glucose
    Concentration (μmol/g)1.27 ± 0.041.72 ± 0.08*0.35 ± 0.040.4 ± 0.04
    C1 (%E)n.d.n.d.n.d.n.d.
Lactate
    Concentration (μmol/g)2.2 ± 0.22.3 ± 0.21.9 ± 0.53.4 ± 0.3*
    C3 (%E)2.3 ± 0.22.6 ± 0.23.4 ± 0.51.2 ± 0.4*
Aspartate
    Concentration (μmol/g)2.9 ± 0.12.7 ± 0.13.3 ± 0.53.9 ± 0.5*
    C3 (%E)7.1 ± 0.86.5 ± 0.65.9 ± 0.46.5 ± 0.8
Glutamate
    Concentration (μmol/g)10.8 ± 0.511.5 ± 0.611.5 ± 0.612.1 ± 0.8
    C2 (%E)2.8 ± 0.33.2 ± 0.3
    C3 (%E)7.7 ± 0.47.6 ± 0.58.7 ± 0.67.7 ± 0.7
    C4 (%E)10.5 ± 0.310.8 ± 0.513.9 ± 0.614.3 ± 1
Glutamine
    Concentration (μmol/g)6.9 ± 0.36.6 ± 0.37.2 ± 0.47.2 ± 0.6
    C2 (%E)7.3 ± 0.46.7 ± 0.3
    C3 (%E)11.2 ± 0.710.4 ± 0.211.5 ± 0.410.0 ± 0.6
    C4 (%E)23.7 ± 1.022.9 ± 0.827.8 ± 0.529 ± 0.8
GABA
    Concentration (μmol/g)1.6 ± 0.11.7 ± 0.21.8 ± 0.22.7 ± 0.4
    C2 (%E)9.2 ± 0.410.3 ± 0.512.0 ± 0.311.3 ± 1
    C3 (%E)5.3 ± 0.75.9 ± 0.47.3 ± 0.65.3 ± 1.4
    C4 (%E)6.9 ± 0.76.1 ± 0.39.2 ± 1.17.3 ± 1.2
  • The natural abundance of13C (1.1%) was subtracted from the percentage enrichments.

  • *P< 0.05 3dRH vs. control;

  • P< 0.05;

  • P< 0.01 hypoglycemia vs. euglycemia within a group. n.d., non-detectable.