TABLE 4

Relative gene expression of novel candidate diabetic nephropathy susceptibility genes in primary human cell lines

Cell lineCPVL Ct valuesCHN2 Ct valuesFRMD3 Ct valuesCARS Ct values
Endothelial cells, iliac17.3 ± 0.712.5 ± 0.112.5 ± 0.38.4 ± 0.4
Fibroblasts15.7 ± 0.1n.e.11.9 ± 0.76.5 ± 0.4
Mesangial cellsn.e.n.e.12.9 ± 0.57.2 ± 0.1
Proximal tubules8.6 ± 0.113.1 ± 0.110.2 ± 0.28.0 ± 0.1
  • The relative gene expression of CPVL, CHN2, FRMD3, and CARS was determined in four primary human cell lines using real-time PCR. Relative gene expression of each gene was calculated in reference to a normalization control (β-actin) and is presented as the mean ΔCt (Ctvalue from each gene minus Ct value from the normalization control) and SD from three separate experiments. Mean ΔCt = 0 equals high relative gene expression (i.e., expression similar to β-actin). n.e., not expressed (mean ΔCt >40.0).