TABLE 1

Effect of TNF-α, IL-6, and resistin on mRNA levels of macrophage-related genes in preadipocytes

BasalTNF-α
IL-6
Resistin
MeanP valueMeanP valueMeanP value
GM-CSF1.0 ± 0.050 ± 290.0041.0 ± 0.1NS1.1 ± 0.1NS
IL-1β1.0 ± 0.0305 ± 1480.0031.7 ± 0.4NS1.3 ± 0.2NS
MIP-1α1.0 ± 0.0804 ± 4320.0030.9 ± 0.2NS1.1 ± 0.2NS
TNF-α1.0 ± 0.03.3 ± 0.40.0031.1 ± 0.2NS1.5 ± 0.5NS
CD681.0 ± 0.03.4 ± 0.90.031.0 ± 0.1NS1.1 ± 0.2NS
MMP31.0 ± 0.059 ± 390.0032.1 ± 0.8NS1.0 ± 0.1NS
  • Data are means ± SE where basal is set to 1. Human preadipocytes in the stromal vascular fraction were cultured for 10 days with or without 5 ng/ml TNF-α, 20 ng/ml IL-6, or 50 ng/ml human resistin. n = 11.