TABLE 1

Metabolic and plasma parameters in wild-type and AMPKα2−/− mice

Metabolic parametersWild-typeAMPKα2−/−
ChowcHFcHF+FChowcHFcHF+F
Food consumption (kJ/g · day)2.0 ± 0.11.8 ± 0.11.9 ± 0.02.0 ± 0.11.8 ± 0.12.0 ± 0.1
Initial body weight (g)27.8 ± 0.527.9 ± 0.527.7 ± 0.527.1 ± 0.327.7 ± 0.427.3 ± 0.4
Body weight gain (g)1.4 ± 0.27.0 ± 1.1*2.8 ± 0.71.8 ± 0.34.6 ± 0.7*1.2 ± 0.4
Adiposity
Epididymal AT (g)0.43 ± 0.021.52 ± 0.19*1.12 ± 0.14*0.37 ± 0.031.19 ± 0.16*0.64 ± 0.07*
Subcutaneous AT (g)0.20 ± 0.010.54 ± 0.05*0.42 ± 0.04*0.17 ± 0.010.34 ± 0.03*0.23 ± 0.01
Epididymal adipocytes size (μm2)ND15,971 ± 1,78410,232 ± 185ND13,298 ± 1,6328 593 ± 896
Subcutaneous adipocytes size (μm2)2,916 ± 6106,775 ± 1,718*7,311 ± 1,308*3,395 ± 1396,625 ± 926*5,432 ± 648*
Plasma metabolites
Triglycerides (mmol/l)1.17 ± 0.081.23 ± 0.110.62 ± 0.07*1.04 ± 0.061.22 ± 0.080.73 ± 0.06*
NEFAs (mmol/l)0.90 ± 0.050.94 ± 0.050.59 ± 0.04*0.88 ± 0.040.99 ± 0.060.68 ± 0.04*
Cholesterol (mmol/l)2.25 ± 0.084.12 ± 0.25*3.10 ± 0.20*2.10 ± 0.063.94 ± 0.18*2.75 ± 0.14*
Glucose (mmol/l)9.9 ± 0.410.7 ± 0.510.4 ± 0.39.7 ± 0.410.0 ± 0.49.4 ± 0.4
Plasma hormones
Insulin fed (ng/ml)0.66 ± 0.111.73 ± 0.29*1.47 ± 0.28*0.60 ± 0.071.34 ± 0.20*0.95 ± 0.12*
Insulin fasted (ng/ml)0.13 ± 0.010.39 ± 0.06*0.19 ± 0.050.16 ± 0.020.22 ± 0.030.15 ± 0.01
Total adiponectin (A.U.)1.15 ± 0.090.97 ± 0.101.33 ± 0.090.82 ± 0.090.80 ± 0.070.97 ± 0.08
HMW: total adiponectin0.38 ± 0.020.36 ± 0.020.44 ± 0.020.35 ± 0.020.34 ± 0.020.39 ± 0.02
  • Data are means ± SE of 27–30 mice for metabolic parameters and 13–15 mice for other measures. AMPKα2−/− and wild-type mice were fed either a Chow diet, cHF, or cHF+F for nine weeks. Food consumption was measured weekly for nine weeks. Body weight gain (see supplementary Fig. 1) and plasma parameters were assessed in ad libitum-fed mice after nine weeks. Plasma insulin levels were also assessed in fasted mice after eight weeks. AT, adipose tissue; A.U., arbitary units; HMW, total adiponectin, ratio of high molecular weight to total adiponectin (for levels of all molecular weight forms of adiponectin; ND, no data; see supplementary Fig. 2);

  • *P < 0.05 vs. genotype Chow;

  • P < 0.05 vs. genotype cHF;

  • P < 0.05 vs. wild-type on respective diet.