TABLE 1

20S proteasome modification after incubation with MGO in vitro

SubunitPeptideSequenceModification
β5117–123LLARQCR7:methylimidazolone
β5124–130IYELRNK5:methylimidazolone
β230–41DDHDKMFKMSEK8:carboxyethyllysine
β269–85MRNGYELSPTAAANFTR17:methylimidazolone
β4217–227CMRVLYYRDAR8:arg-pyrimidin
β4220–231VLYYRDARSYNR5:methylimidazolone
β4225–240DARSYNRFQIATVTEK7:methylimidazolone