TABLE 1

Rat body weight, blood glucose levels, and glycated hemoglobin at end point

nWeight (g)Blood glucose (mmol/l)A1C (%)
Cohort 1 (12 weeks)
    Control5603.7 ± 38.8****8.14 ± 1.52****4.34 ± 0.24****
    Diabetic5280.4 ± 16.528.16 ± 3.537.70 ± 0.78
Cohort 2 (16 weeks)
    Control11679.4 ± 25.3****8.80 ± 2.34****4.76 ± 0.21****
    Diabetic7367.7 ± 42.531.09 ± 2.968.90 ± 0.73
Cohort 3 (22 weeks)
    Control6756.17 ± 70.03*8.25 ± 0.85*3.48 ± 0.10*
    Diabetic6413.25 ± 64.3030.72 ± 1.657.76 ± 0.77
    Diabetic + insulin6498.97 ± 24.02***20.37 ± 11.11***7.32 ± 0.61
Cohort 4 (22 weeks)
    Control12782.73 ± 107.39*8.29 ± 1.58*4.65 ± 0.38*
    Diabetic12390.13 ± 43.7229.03 ± 2.6310.68 ± 1.04
    Diabetic + insulin12493.30 ± 50.38**20.23 ± 6.859.10 ± 0.65**
  • Starting body weight ranged between 275 and 325 g. Cohorts were maintained for 12–22 weeks. Rats from cohort 3 and 4 were used for the preparation of lumbar DRG tissue homogenate samples and isolated mitochondria samples from lumbar DRG, respectively. Values are means ± SD.

  • *P < 0.05 vs. other groups;

  • **P < 0.001 vs. diabetic;

  • ***P < 0.05 vs. diabetic (all by one-way ANOVA with Tukey's post hoc test); and

  • **** P < 0.0001 vs. diabetic (Student's t test). Note that %A1C values in cohort 3 were derived using the DCA 2000 + kit (n = 4–5) and for cohort 4 were generated using the A1C Now kit (n = 8–11).