Quantitative stereological analyses of the endocrine pancreas of GIPRdn transgenic pigs (tg) and wild-type control pigs (wt)
| Parameter | 11 weeks (n = 5 wt, 5 tg) | 5 months (n = 4 wt, 4 tg) | 1–1.4 years (n = 5 wt, 5 tg) | ANOVA | ||
|---|---|---|---|---|---|---|
| Means ± SE | Means ± SE | Means ± SE | Group | Age | Group × age | |
| Pancreas weight (g) | ||||||
| Wild type | 34.5 ± 4.2 | 115.3 ± 5.6 | 183.4 ± 13.8 | NS | <0.0001 | NS |
| Transgenic | 32.7 ± 3.1 | 125.7 ± 6.1 | 206.1 ± 4.9 | |||
| VV(β-cell/islet) (%) | ||||||
| Wild type | 69.8 ± 2.2 | 89.0 ± 1.5 | 90.2 ± 1.2 | 0.0066 | <0.0001 | 0.0024 |
| Transgenic | 70.8 ± 1.3 | 87.0 ± 1.2 | 76.4 ± 3.2* | |||
| VV(α-cell/islet) (%) | ||||||
| Wild type | 14.1 ± 1.2 | 5.0 ± 0.8 | 5.0 ± 0.7 | 0.0122 | <0.0001 | 0.0008 |
| Transgenic | 12.2 ± 0.7 | 6.5 ± 0.7 | 13.8 ± 2.3* | |||
| VV(δ-cell/islet) (%) | ||||||
| Wild type | 13.5 ± 2.8 | 4.3 ± 0.8 | 2.2 ± 0.6 | NS | <0.0001 | NS |
| Transgenic | 13.0 ± 1.0 | 5.5 ± 0.9 | 5.8 ± 0.9† | |||
| VV(pp-cell/islet) (%) | ||||||
| Wild type | 2.7 ± 0.7 | 1.8 ± 0.3 | 2.8 ± 0.8 | NS | 0.0355 | NS |
| Transgenic | 3.9 ± 0.5 | 1.3 ± 0.3 | 3.8 ± 1.1 | |||
| V(β-cell/islet) (mm3) | ||||||
| Wild type | 168.7 ± 29.5 | 1,088.2 ± 82.0 | 1,694.6 ± 251.7 | 0.0002 | <0.0001 | 0.0024 |
| Transgenic | 152.1 ± 17.1 | 664.1 ± 74.5† | 663.7 ± 130.5* | |||
| V(α-cell/islet) (mm3) | ||||||
| Wild type | 32.6 ± 8.7 | 58.4 ± 6.3 | 95.7 ± 17.4 | NS | <0.0001 | NS |
| Transgenic | 24.5 ± 1.7 | 47.7 ± 4.5 | 112.8 ± 14.5 | |||
| V(δ-cell/islet) (mm3) | ||||||
| Wild type | 26.6 ± 3.9 | 49.5 ± 6.1 | 36.8 ± 6.3 | NS | 0.0014 | NS |
| Transgenic | 25.9 ± 1.8 | 39.6 ± 3.5 | 47.5 ± 5.2 | |||
| V(pp-cell/islet) (mm3) | ||||||
| Wild type | 6.0 ± 1.8 | 20.7 ± 2.9 | 52.4 ± 12.6 | NS | <0.0001 | NS |
| Transgenic | 8.2 ± 1.7 | 9.3 ± 2.1 | 30.3 ± 7.9 | |||
Data were analyzed by the general linear models procedure (SAS Institute) taking the effects of group (wild type, transgenic), age (11 weeks, 5 months, 1–1.4 years), and the interaction group × age into account. For significant effects of these factors P values are indicated in the last three columns. In addition, significant differences between groups within age classes are marked by:
*P < 0.01;
†P < 0.05;
NS, not significant.