TABLE 1

Biochemical properties and immunohistologic analyses in renal glomeruli of normal and diabetic rats and in diabetic rats subjected to DAPT treatment

Normal ratsDiabetic rats
Vehicle5 mg/kg DAPT10 mg/kg DAPT
Biochemical properties
    Blood glucose82 ± 1.6407.2 ± 10.6*400.7 ± 7.1*397.4 ± 10*
    A1C4.2 ± 0.19.6 ± 0.8*10.3 ± 0.3*9.5 ± 0.5*
    Urinary protein secretion0.57 ± 0.134.73 ± 2.63*1.07 ± 0.351.08 ± 0.2
Immunohistomorphormetry
    Jagged-110.4 ± 0.426.3 ± 0.1*22.9 ± 0.9*22.4 ± 0.9*
    NICD4.9 ± 0.217.5 ± 0.7*7.4 ± 0.3*3.7 ± 0.2
    HIF-13.2 ± 0.132.17 ± 1.3*9.8 ± 0.4*9.4 ± 0.4*
    VEGF13.8 ± 0.730.9 ± 1.3*23.5 ± 1.1*17.3 ± 1*
    Nephrin20.6 ± 1.28.2 ± 0.8*13.7 ± 0.8*18.1 ± 0.6
    CD2AP12.1 ± 0.516.5 ± 0.715.9 ± 0.714.6 ± 0.6
    Podocin16.1 ± 0.716.9 ± 0.718.2 ± 0.719.8 ± 0.8
TUNEL apoptosis12.3 ± 1.035.2 ± 1.8*25.9 ± 1.2*16.8 ± 0.9
  • For the biochemical properties, the data represent means ± SE calculated from six rats. Glucose (milligrams per deciliter) and A1C (milligrams per deciliter) were measured from tail vein blood. Total urinary protein secretion (milligrams per milligram creatinine) was assayed using urinary protein kits and normalized to total creatinine concentrations in urine. For the immunohistologic analyses, the data are means ± SE calculated from the percentage of positively stained cells and total cells in each image. Six random images from three sections obtained from each rat were randomly selected and analyzed. P < 0.05 for

  • *normal mice,

  • †diabetic/vehicle mice, and

  • ‡diabetic mice treated with DAPT (5 milligrams per kilogram).