TABLE 1

Metabolic parameters of C57Bl/6 mice fed with normal diet or HFD for 8 weeks

FedFasted
Normal dietLDRHDRNormal dietLDRHDR
Weight gain (g)8.5 ± 0.2 (62)15.5 ± 0.2*** (60)20.4 ± 0.2***,§§§ (72)5.5 ± 1.0 (5)16.9 ± 0.6*** (5)21.0 ± 0.3***,§§ (5)
Insulin (pmol/l)252 ± 24 (59)266 ± 20 (58)658 ± 54***,§§§ (67)133 ± 87 (5)338 ± 84 (5)503 ± 40* (5)
ProIns (pmol/l)18.5 ± 2.3 (8)23.7 ± 1.9 (9)31.8 ± 4.0* (9)NDNDND
ProIns/Ins (%)7.8 ± 1.3 (8)9.4 ± 1.4 (9)5.5 ± 0.9§ (9)NDNDND
FFA (mmol/l)0.60 ± 0.04 (33)0.55 ± 0.03 (33)0.58 ± 0.03 (38)0.77 ± 0.06 (5)1.12 ± 0.06 (5)1.58 ± 0.30* (5)
TG (mmol/l)0.41 ± 0.02 (33)0.32 ± 0.01*** (34)0.33 ± 0.01*** (38)0.36 ± 0.04 (5)0.40 ± 0.04 (5)0.39 ± 0.03 (5)
TC (mmol/l)1.50 ± 0.07 (8)1.90 ± 0.14 (8)2.58 ± 0.21***,§ (8)NDNDND
  • Data are means ± SE of n animals as indicated in parentheses. Plasma triglyceride (TG), FFAs, TC, proinsulin (ProIns), and insulin (Ins) were determined in anesthetized overnight fasted and/or fed male mice on a high-fat diet (LDR and HDR) or normal diet for 8 weeks. Delta body weight represents the weight gain of mice since the introduction of diets (normal diet or HFD) at 6 weeks of age. LDR or HDR versus normal diet:

  • *P < 0.05,

  • **P < 0.01,

  • ***P < 0.001; HDR versus LDR:

  • §P < 0.05,

  • §§P < 0.01,

  • §§§P < 0.001. One-way ANOVA–Bonferroni multiple comparison test. ND, not determined.