TABLE 3

Circulating lipids and hormones in caloric-restricted ob/ob mice with leptin treatment

Treatment groupFFAs (mM)Triglycerides (mg/dL)Adiponectin (μg/mL)Leptin (ng/mL)
Wt RF0.7 ± 0.1 (4)45.5 ± 5.2 (3)10.6 ± 1.2 (5)2.6 ± 0.5 (4)
Wt CR1.0 ± 0.1c (5)43.3 ± 4.8c (7)18.0 ± 1.4a,b,c (5)3.0 ± 0.2 (4)
Wt CR + L 1 week0.8 ± 0.2c (4)32.0 ± 1.9c (4)13.8 ± 1.3c (5)6.4 ± 1.9a (3)
ob/ob RF0.7 ± 0.1 (3)40.5 ± 2.0 (3)10.7 ± 1.4 (4)ND (3)
ob/ob CR1.4 ± 0.02a,b (5)57.6 ± 3.6b (5)21.9 ± 0.6a,b (6)ND (3)
ob/ob CR + L 1 week0.6 ± 0.1c (4)50.5 ± 2.6 (4)17.9 ± 2.0a,b,c (4)1.0 ± 0.8 (3)
Wt CR intracerebroventricular saline1.1 ± 0.20 (5)28.6 ± 1.5 (3)14.3 ± 1.3 (4)3.3 ± 0.9 (3)
Wt CR intracerebroventricular leptin0.1 ± 0.04a,c (6)17.6 ± 2.5c (6)8.6 ± 1.6a,c (5)3.5 ± 0.8 (3)
ob/ob CR intracerebroventricular saline1.6 ± 0.30a (5)43.8 ± 3.3a (4)23.6 ± 1.5a (6)ND (3)
ob/ob CR intracerebroventricular leptin0.6 ± 0.05a,c (5)32.6 ± 3.5c (4)37.9 ± 2.0a,c (7)ND (3)
  • Circulating concentrations of FFAs, triglycerides, adiponectin, and leptin were measured in intraperitoneal (L 1 week)- and intracerebroventricular-treated mice after 6 h of fasting (number of animals is indicated in parentheses).

  • aP < 0.05 vs. wt RF or wt CR intracerebroventricular saline;

  • bP < 0.05 vs. ob/ob RF;

  • cP < 0.05 vs. ob/ob CR or ob/ob CR intracerebroventricular saline. L 1 wk, 1 week of leptin treatment; ND, not detected; RF, random fed; wt, wild type.