Table 1

Effects of significant hits identified in a primary screen for β-cell dysfunction

Gene	Locus	Low glucose	High glucose	IBMX	Tolbutamide	Cell count
ABCC8	KCNJ11	48.2^*	24.0	26.7^*	1.8	−1.4
ADAMTS9	ADAMTS9	6.3	−4.8	2.8	−8.0	12.2^*
ADIPOQ	ST64GAL1	87.0^*	23.2	23.1	8.8	−7.3
ARL15	ARL15	−5.5	−25.9^*	−2.1	−15.5	−1.5
BCAR1	BCAR1	5.9	25.2	28.5^*	9.5	−7.0
BCL6	LPP	−20.7^*	−8.9	−1.0	−12.0	5.6
BMP8B	MACF1	7.8	16.5	9.5	26.9^*	−1.0
CCNT2	TMEM163	−32.6^*	1.8	4.7	5.0	−2.8
CDKAL1	CDKAL1	2.4	1.7	−10.6	−23.5^*	7.5
DGKQ	MAEA	3.3	17.5	20.4	33.6^*	−9.8
DMRTA2	FAF1	32.3^*	24.5	13.1	22.7	−0.3
ELAVL4	FAF1	−3.6	9.1	21.2	22.8	−11.4^*
ETV5	IGF2BP2	−12.4	−25.6^*	−10.9	−12.6	−2.5
FAH	ZFAND6	−23.1^*	−20.6^*	−16.9	−27.2	−2.3
FBXW7	TMEM154	45.2^*	9.6	8.7	11.7	9.9^*
GINS4	ANK1	−16.1	−14.2	−9.5	−17.0	8.7^*
GLIS3	GLIS3	−13.0	−10.6	6.4	−9.3	−10.3^*
HEYL	MACF1	29.2	29.2^*	0.4	20.0	−7.2
HMGA2	HMGA2	16.5	4.1	14.2	24.1^*	−1.2
HNF1A	HNF1A	21.7	38.3^*	23.1	25.5^*	5.2
HNF4A	HNF4A	36.7^*	66.9^*	74.9	92.9^*	−8.8
IGF2	DUSP8	−26.3^*	−10.4	1.2	0.1	−1.3
INS	DUSP8	−53.5^*	−44.8^*	−48.7	−38.7^*	0.9
KCNK17	KCNK16	−9.4	−17.3	−2.5	−1.2	9.8^*
KCTD15	PEPD	21.7	9.4	12.5	0.8	−10.9^*
KIF11	HHEX/IDE	45.4^*	35.0^*	26.9	55.4^*	−40.1^*
LINGO1	HMG20A	0	19.1	−12.4	−14.9	7.9^*
MFGE8	AP3S2	30.0^*	3.4	2.8	−1.7	−5.7
MIER3	ANKRD55	5.9	36.5^*	5.8	18.2	1.9
NDUFS4	ARL15	1.6	−4.9	3.9	−1.7	10.8^*
PABPC1L	HNF4A	−9.7	−12.3	−10.4	−28.8^*	−1.2
PHF23	SLC16A11	−25.6^*	−1.7	−5.9	3.5	−0.9
PLA2R1	RBMS1	8.6	1.6	10.8	1.6	9.1^*
PRDX3	GRK5	24.0	31.7^*	23.4	9.6	16.6^*
PTHLH	KLHDC5	−2.8	−6.5	−0.5	−25.0^*	−5.9
RND3	RND3	−8.7	−6.1	0	−3.0	−14.9^*
SLC2A4	SLC16A11	14.5	0	27.2^*	18.2	−1.6
SOCS7	HNF1B	3.9	−18.5^*	11.2	−14.7	−1.6
SPPL3	HNF1A	−11.9	−21.9^*	−6.1	−10.0	−10.8^*
STK38L	KLHDC5	15.2	40.9^*	4.7	25.2^*	−3.0
THADA	THADA	−1.9	6.5	27.5	24.8^*	−10.3
TLE1	TLE1	4.3	−5.0	−23.0^*	16.2	4.6
TM6SF2	CILP2	−22.6^*	−8.3	−0.8	−12.0	−8.7
UPF2	CDC123	7.5	−12.5	4.7	−24.9^*	−3.2
ZMIZ1	ZMIZ1	−29.5^*	−21.4^*	−19.8	−16.8	−15.2^*

The table lists effect sizes (% deviation from NT control) for each gene with a least one significant effect across the five phenotypes measured. All insulin secretion measurements were normalized on a per-well basis to cell counts, and the mean percentage deviations from NT control were then calculated for each condition. For cell counts, values were median-normalized for interplate differences, and the mean percentage deviations from NT control were calculated across conditions.
*q < 0.05 by Student t test (FDR-adjusted).